New probe of lanthanide complex for detection some of nucleobase, nucleosides and nucleotides, by the luminescent Tb (III) - 8-allyl-2-oxo-2 H-chromene-3-carbaldehyde probes

Hassan A. Azab, Gasser M. Khairy , N. Abd El-Ghany, Marwa. A.Ahmed


The luminescence arising from lanthanide cations offers several advantages over organic fluorescent molecules sharp, distinctive emission bands allow for easy resolution between multiple lanthanide signals; long emission lifetimes (μs–ms) make them excellent candidates for time resolved measurements and high resistance to photo bleaching allow for long or repeated experiments.  A developed method is presented for determination of nucleobase, nucleosides and nucleotides using the effect of quenching of fluorescence of the easily accessible terbium (III) - 8-allyl-2-oxo-2H-chromene-3-carbaldehyde (AOCC) complex in 1:2 metals: ligand ratio. The interaction of terbium (III) - 8-allyl-2-oxo-2H-chromene-3-carbaldehyde (AOCC) complex with nucleosides (NS) (cytidine, inosine), nucleobase (NB) (adenine), nucleotides (NT) (5′-GMP), (5′-IMP), (5′- ATP), (5′-CMP) and (5′-ADP) has been studied using normal luminescence techniques. The linear ranges for determination of the selected biomolecules are 1.6–36.6 µ, and with limit of detection were in the range of 0.906- 8.28µ

Keywords: Fluorescence, Nucleotides, Nucleobases, Nucleosides, Lanthanide complexes, time-resolved.

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