Protective Effect of Ginger (Zingiber officinale) Extract on Gasoline-induced Oxidative Stress in Albino Rats

Heba Nageh Gad El-Hak, Mahmoud Ezzat Mohallal, Nour El-Din Hussein Saleh, Mohamed Salah El-Deen El-Naggar

Abstract

ABSTRACT

There were many attempts to find a way to protect humanity from leaded gasoline pollution which causing harmful effects to the human health, generating massive amount of reactive oxygen species in body organs including eyes. The present study mainly aimed to investigate whether ginger (Zingiber officinale) aqueous extract protects rat's eyes from oxidative stress induced by leaded gasoline. Sixty male adult albino rats (120-150 gm) were divided into 10 groups (n=6) inside inhalation chambers. Control group (G1) subsisted without any special treatment. Gasoline groups (G2-G5) inhaled leaded gasoline with nominal concentration of 18.18 ppm for exposure times 3, 6, 9 and 12 hrs/days for 14 days. Ginger group (G6) orally received 100 mg of ginger/kg once/day for 14 days. Protection groups (G7-G10) inhaled gasoline (like groups 2-5) and orally receiving 100 mg/kg ginger once/day for 14 days. After sacrificing animals, lead level, lipid peroxidation (MDA), protein carbonyl (PCC) and glutathione (GSH) content were determined in blood and eyes. The gasoline exposure groups revealed a significant increase in blood-lead level, blood and eye’s contents of both MDA and PCC, and significant decrease in GSH content in blood and eyes. On the other hand, ginger reduced the gasoline effects in blood and eye’s MDA, PCC and blood-lead contents, and elevated GSH level of eyes. Accordingly, ginger may have a remarkable protective role against oxidative stress induced by leaded gasoline exposure.

 


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