Molecular and pharmacological investigation of Stipagrostis scoparia growing in North Sinai

Mayada M. El Ayouty, Hashim A. Hassanean, Rawia Zayed, Mostafa K. Mesbah and Amany K. Ibrahim

Abstract

ABSTRACT

Although many plants of Poaceae family attracted several investigators regarding their pharmacological aspects, there is almost no report on the pharmacology of Stipagrostis scoparia. Accordingly, it was necessary to carry out pharmacological study on the wild Egyptian plant which grows in North Sinai and selectively discriminate the plant using Random Amplified Polymorohic DNA technique. RAPD DNA analysis of the plant was performed using ten decamer primers. It showed distinguishable bands and generated 72 fragment patterns. The analysis of RAPD data can select the use of primers Z-02, C-17 and Z-08 for the selective discrimination of Stipagrostis scoparia growing in Egypt. Concerning the biological studies, the methanolic extract of Stipagostis scoparia exhibited significant analgesic, anti-inflammatory and anti-oxidant activities after in-vivo assays. Results obtained on assessing the long-term anti-hyperglycemic effect revealed a significant reduction in blood glucose level in Alloxan-diabetic rats treated with the methanolic extract. Prolonged administration of the methanolic extract significantly decreased the level of liver enzymes in carbon tetrachloride-intoxicated rats. According to the acute toxicity studies it was found that the methanolic extract of Stipagrostis scoparia is safe up to 7 g/kg b.wt.

Key words: Stipagrostis scoparia; RAPD analysis; analgesic; anti-inflammatory; anti-oxidant


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